Journal: Cell reports

Article Title: MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells

doi: 10.1016/j.celrep.2024.114565

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Article Snippet: Recombinant Mouse IL-4 , R&D Systems , Cat# 404-ML.

Techniques: Purification, Control, Virus, Plasmid Preparation, Expressing, Retroviral, Recombinant, Activation Assay, Modification, Infection, Transfection, Lysis, Extraction, Western Blot, Electron Microscopy, Cell Isolation, Staining, Mutagenesis, Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Gene Expression, cDNA Synthesis, Sequencing, Software, Real-time Polymerase Chain Reaction

Journal: Cell Reports

Article Title: Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming

doi: 10.1016/j.celrep.2018.11.018

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Article Snippet: For evaluation of IL4 in CD4+ T, 100ng/ml mouse IL4 (R&D System) and 10μg/ml anti-mouse-IL12 and anti-mouse IFNγ antibodies (Biolegend) have been added to the cells all the days (th2 polarizing conditions).

Techniques: Purification, Recombinant, Protease Inhibitor, Polymer, Bradford Protein Assay, Flow Cytometry, Cell Isolation, Staining, ATP Assay, Detection Assay, Membrane, In Situ, RNA Sequencing, Gene Expression, Transgenic Assay, Plasmid Preparation, Software, Live Cell Imaging, Microscopy, Diagnostic Assay, Transfection